Development and Electrical Analysis of DNA Aqueous Solution on Microchannel Biochip
A microchannel biochip employed by micro-electro-mechanical systems (MEMS) and based on Si substrate was designed and developed in the research. The ionic CH3COOH or DNA aqueous solutions were filled in the microchannel for the reagents in the experiment and the DC electrical characterization of the samples was carried out and analyzed when applying voltage in the microchannel. Furthermore, the curve of current versus voltage of these reagents was measured precisely by a high-resolution measurement system. In the experiments, it was successfully to distinguish the reagents as dATP, dGTP, dTTP, and dCTP. Moreover, the curves displayed oscillation phenomena with time because the accumulating charges generated a diffusion effect to counteract the electrical drift effect caused by applying voltage. The initial results demonstrated that the designed microchannel biochip is feasible to be used and distinguish different DNA molecules. The method should be beneficial for the advanced studies and applications of biomedical diagnostic technology.